ABSTRACT
Highly reactive oxyradicals and electronically excited triplet carbonyls can be generated in vitro by iron complexes and heme enzyme-catalyzed aerobic oxidation of synthetic or naturally occurring substances capable of enolization in aqueous medium. Monoenols and enamines, obtained by (alpha-methyne-carbonyl and -imine enolization, undergo dioxygen insertion and ultimately originate triplet species; e.g., isobutanal, 3-methylacetoacetone, Schiff bases. In turn, (alpha-hydroxy- and (alpha-aminocarbonyls (e.g., carbohydrates, 5-aminolevulinic acid) tautomerize to enediols and enolamines and yield oxyradicals, initiated by electron transfer to dioxygen, as polyphenols (e.g., 6-hydroxydopamine) and polyphenolamines do. Free radicals and excited species have been implicated in several normal and pathological processes. We here briefly review our contributions to this research area, emphasizing a possible in vivo prooxidant role for 5-aminolevulinic acid, the heme precursor accumulated in several porphyric disorders (e.g., lead poisoning, acut intermittent porphyria, tyrosinosis).
Subject(s)
Animals , Humans , Aminolevulinic Acid/chemistry , In Vitro Techniques , Reactive Oxygen Species , Free Radicals , Imino Acids/metabolism , Iron/metabolism , Lead/metabolismABSTRACT
Estudou-se a incidência de infecçöes hospitalares e o consumo de antibióticos no Hospital do Servidor Público Municipal de Säo Paulo, relativos a 7.197 doentes internados no período de 6 meses (novembro 1983 a abril de 1984), encontrando incidência média mensal de 2,8%. Observou-se diminuiçäo no consumo de antimicrobianos (20,6%) no transcorrer do período de estudo, apesar do aumento verificado no número de internaçöes. O estudo dos gastos com internaçäo mostrou uma economia acumulada de Cr$26.378.619, equivalentes a 2.577 ORTN no período ou 429,5 ORTN mensais